NAD+

What is NAD+?

NAD+ is not a peptide. It is a dinucleotide: two nucleotides, one built around an adenine base and the other around a nicotinamide group, joined through a pair of phosphate groups. Chemically it is the dinucleotide of adenosine monophosphate and nicotinamide mononucleotide, with the formula C21H27N7O14P2, a molecular weight of 663.4 g/mol and CAS number 53-84-9. It contains no amino acids and no peptide bonds, which places it in a different chemical class from the peptides elsewhere in the Celyfe range.

NAD+ works as an electron carrier. In its oxidised form it accepts a hydride ion during glycolysis and the citric acid cycle and becomes NADH, its reduced form. NADH then passes those electrons to the electron transport chain in mitochondria, where the flow is coupled to ATP production, and NAD+ is regenerated. Because the two forms are interconverted continuously, researchers often use the NAD+/NADH ratio as a readout of a cell's redox and metabolic state.

NAD+ is also consumed, not just cycled, by three enzyme families that are widely studied in cell biology: sirtuins (NAD+-dependent deacylases), poly(ADP-ribose) polymerases (PARPs) and the CD38/CD157 glycohydrolases. Research on these enzymes uses defined NAD+ concentrations as the controlled variable, which is why NAD+ appears in a research catalogue alongside peptides.

How NAD+ is used in the Celyfe system

The NAD+ Precision Pen holds 500mg of NAD+ in a pre-filled, pre-mixed 3ml cartridge, a supplied concentration of 166.7mg/ml. The Celyfe precision pen dispenses 0.01ml per click, so each click carries 1.667mg of NAD+ and a full cartridge gives 300 calibrated clicks. The vehicle also contains a phosphatidylcholine complex (50mcg) and trans-resveratrol (25mcg); both are listed on the label. The published batch certificate reports NAD+ only.

The pen ships already in solution, buffered and sealed, so there is no powder, no bacteriostatic water and no reconstitution step. That matters for NAD+ specifically: the compound is sensitive to pH, temperature and light in solution, and reconstitution is the moment where a redox cofactor is most easily degraded. The trade-off is the cold chain. The pen must be refrigerated on arrival and never frozen.

The published certificate for batch A26085 is an HPLC report from a third-party laboratory, Analiza Białek in Wrocław, on a sample Celyfe supplied, completed on 2 October 2026. It reports 99% chromatographic purity and a measured content of 182.93mg/ml, about 110% of the 166.7mg/ml label: purity is the share of detected material that is NAD+, while content is how much NAD+ is in each ml compared with the label. The certificate is in the COA library. A reusable Celyfe metal pen is added free to every order, so you pay only for the cartridge.

What the research literature covers

The NAD+ Precision Pen product page lists three review articles as its literature references. One, in Nature Reviews Molecular Cell Biology, covers NAD+ metabolism and its roles in cellular processes during ageing. A second, in Science, surveys NAD+ in ageing, metabolism and neurodegeneration. The third, in Cell Metabolism, reviews the in vivo evidence on NAD-boosting molecules. The journal article on understanding NAD+ decline summarises the same field for a general reader.

These are reviews of a research field, not evidence about the Celyfe pen, and nothing on this page is a claim about effect in a human or animal body. The pen is supplied and labelled for in-vitro laboratory research.

Handling, storage and quality

Because the cartridge is already in solution, the correct storage condition is refrigeration between 2 and 8 degrees Celsius, never freezing. Freeze-thaw cycling damages compounds already in solution, which is a different situation from freezing dry powder. Keep the cartridge sealed and away from light, prime the pen before first use to clear air from the needle path, and handle under standard aseptic practice.

Orders ship cold-chain in a sealed foil pouch with ice packs inside a plain box, packed to hold temperature for about a week. Orders are dispatched within 1 to 2 working days with tracked delivery, and worldwide delivery typically takes 3 to 5 working days in transit. Refrigerate the pen as soon as the parcel arrives.

Buying NAD+ in the UK: what to check

NAD+ is lawful to buy and possess in the UK for laboratory research. It is not a licensed medicine and the Celyfe pen is supplied for in-vitro research use only. The product page suggests checking the following before ordering from any supplier.

• Mass per cartridge and the concentration it implies, not just a headline number.
• A batch-specific certificate of analysis naming the batch and the analytical method, viewable before you order.
• Format: powder needs bacteriostatic water and a reconstitution step; a pre-mixed pen needs cold-chain delivery and a refrigerator.
• What is in the box: the Celyfe pen is the device and the cartridge together, not a bare vial.
• Documentation: Celyfe publishes third-party HPLC certificates for the NAD+ and WOLVERINE pens in the COA library; GLOW and KLOW certificates are being re-issued.

Related compounds and comparisons

Two other compounds travel in the same cartridge. Phosphatidylcholine is the phospholipid carrier in the vehicle, and resveratrol is the stilbenoid polyphenol included alongside it. The comparison page NAD+ vs Resveratrol explains why they are read together rather than as alternatives: NAD+ is the coenzyme itself, resveratrol is a sirtuin activator on the same pathway.

NAD+ precursors such as NMN and NR are discussed in the journal, in NMN vs NR and in the NAD+ pens guide, which sets out why Celyfe supplies the coenzyme directly instead of a precursor. For the peptides in the wider range, the GLOW, KLOW and WOLVERINE pens are on sale now and each has its own compound page here.